. . . . "Each pre-registered UniProt query was fetched once (size 50, accession ascending, release 2026_03 from the X-UniProt-Release header). Sequences outside length 80–400, sequences with nonstandard letters, and later sequences of a genus already kept were dropped, leaving 12 sequences per domain. Archaeal rplB returned an empty result, so the pre-registered second symbol rpl2 was used. Cross-domain Smith-Waterman identity (BLOSUM62, gap open 11, gap extend 1) was compared with one composition-matched shuffle of the second sequence per pair. A family passes only if all three domain pairs have median identity above 0.20 and above the shuffle 95th percentile. NCBI dataset_report total_count values were stored and not used. The UniProt help/license page was an HTML shell with no readable licence line, so the licence is null." . . "Measured: 0 scorable ribosomal families whose three cross-domain pairs each beat the shuffle bar (n = 3, 0 of 3 families passed; 0 of 9 domain pairs passed). Interpretation: Darwin's sentence is a historical inference about every organism that has ever lived. The numbers decide only whether three ribosomal proteins in extant bacteria, archaea and eukaryotes are more alike across domains than shuffled sequences of the same composition. They do not decide that those organisms descended from one primordial form, and they do not decide anything about lineages that left no sequences, viruses, or a single tree of life. Congruent pairwise identity is a homology observation, not a genealogy." . "Claim test of darwin1859-ch14-04: refutes, confidence 0.46" . . . "Reviewed protein sequences with gene symbols and taxonomy for the three domains of extant cellular life, public and reusable, plus a composition-matched shuffle computed in this script. A tree or a count of genomes does not by itself decide the verdict. No dataset of organisms that left no descendants is admissible, and none is required, because that clause is not scored." . "claim-test/0.1.0" . "biological" . "For at least two of those proteins, a cross-domain comparison fails that bar." . "All nine cross-domain comparisons missed the bar. In eight of the nine, the median real identity was still above the median shuffle identity. Local-alignment percent identity can be high on a short chance match, so the shuffle 95th percentile is a harsh bar. This pattern is not evidence of multiple origins." . "Archaeal uL2 used gene symbol rpl2 because the first pre-registered symbol, rplB, returned no results. Symbols were not changed after the identities were computed." . "NCBI assembly totals (Bacteria 4094585, Archaea 55463, Eukaryota 74855) are context only. The UniProt licence page returned an HTML shell, so no licence was recorded." . "The sample is twelve genera per domain, taken from the fifty lowest accessions in UniProt release 2026_03. It is not a tree, and it is not a sample of all extant cellular life. Viruses and extinct lineages were not included." . "The verdict refutes only the pre-registered pairwise-identity prediction for this sample of extant sequences. It does not test, and does not refute, the historical claim that every organism that ever lived descended from one primordial form." . "For each of uL2, uS3 and uS11, median cross-domain Smith-Waterman identity among extant Bacteria, Archaea and Eukaryota exceeds both 0.20 and the 95th percentile of composition-matched shuffles." . "Pre-registered. REFUTES the extant-homology reading if at least two families are scorable and at least two scorable families fail. A family fails when it is scorable and at least one of its three domain pairs does not pass. MIXED if all three families are scorable and exactly one fails, or if exactly two are scorable and one of them fails. Fewer than two scorable families is inconclusive (underpowered). An unobtainable UniProt search is inconclusive (data-unavailable). A refutation is a refutation of this pairwise-identity prediction in this sample, not a demonstration of multiple primordial origins, and a support is not a test of organisms that left no descendants." . "0.46"^^ . . . "refutes" . "Reviewed UniProt sequences of three ribosomal proteins from extant Bacteria, Archaea and Eukaryota are more similar across domains than composition-matched shuffles of the same sequences. This is a statement about extant cellular life only." . "Per family, three cross-domain medians of local-alignment identity against a seeded shuffle of the second sequence. Support requires all three families scorable and passing. Refutation requires at least two scorable families of which at least two fail. The historical universal is not scored." . "Pre-registered, before any UniProt or NCBI download. Scope: extant cellular life in NCBI taxids 2 (Bacteria), 2157 (Archaea) and 2759 (Eukaryota). The historical sentence, that every organism that ever lived descended from one primordial form, is not given a support or refute statistic. Viruses and extinct lineages with no sequences are outside the sample. Families and the only gene symbols that may be queried, in this try-order, first symbol that yields at least 8 kept sequences wins and symbols are never pooled: uL2 Bacteria rplB, Archaea rplB then rpl2, Eukaryota RPL8; uS3 Bacteria rpsC, Archaea rps3 then rpsC, Eukaryota RPS3; uS11 Bacteria rpsK, Archaea rps11 then rpsK, Eukaryota RPS14. Each query is one GET to https://rest.uniprot.org/uniprotkb/search with query (reviewed:true) AND (taxonomy_id:TAXID) AND (gene_exact:SYMBOL), fields accession,gene_primary,length,sequence,organism_name, format json, size 50, sort accession asc. From that single page, walk accessions ascending, drop a sequence whose length is outside 80 to 400 inclusive or whose sequence uses a letter other than ACDEFGHIKLMNPQRSTVWY, and keep at most one sequence per genus (first token of organism_name) until 12 sequences. A domain is filled only from that page. A family is scorable only if all three domains have at least 8 kept sequences. Cross-domain pairs only. Identity is Smith-Waterman (BLOSUM62, gap open 11, gap extend 1) identical columns divided by identical plus mismatched plus gapped columns in the local alignment. For each pair the second sequence is shuffled once with numpy PCG64 seeded by the first 8 bytes of SHA-256 of the string 20261102|accessionA|accessionB as a little-endian integer. A domain pair passes if its median real identity is greater than the smallest shuffle identity whose empirical cdf is at least 0.95, and the median real identity is at least 0.20. A family passes if all three domain pairs pass. SUPPORTS the extant-homology reading if all three families are scorable and all three pass. NCBI genome dataset_report total_count values for the three taxids, page_size 1, are context only and cannot change the verdict." . "false"^^ . . "reviewed ribosomal-protein sequences for Bacteria, Archaea and Eukaryota" . "" . . "Dataset: UniProtKB reviewed sequences, release 2026_03" . . . . . . . . . . . . "sha256:05647ed6f305fa8a9395c92cf449b8b03c71fa80736903256ebd85c78d25f688" . "sha256:06d446384295244d1df901dd1a9646f07757acfce553e6b8ae40d6625f841146" . "sha256:1ca0e853db1edbf3b12eaeb1c0d856aadd710b9b377ae909d6592624c1e94c14" . "sha256:3fde7df4be12c7aa61abafee70f4dad8e4e826dd5e1ad50739b21809915eebac" . "sha256:5021e624e752b001ce3e3846e8f158ed4aeb93a4c9a72fdb35a0c5b14a0eea84" . "sha256:576638e6694a110ba0035c02d99606a4b13cd35a6f4365989d8c245ee100fb1a" . "sha256:7c34ed7e57ea904c78b813ea0747057702500ba6b00ae0dc0db8f542ed78a0cc" . "sha256:b940a4cf82447e224ce40a5c67b29cc3c55cabcb5e1e671f75b6bb6cdbc211f5" . "sha256:c9b98b0d591592830f51b023b4d8f010131659f662f946a0a8b90081f4cc79e6" . "sha256:ff148dfc88e607c03dc04c051b6b7a7c2bfe2083574e67a17489673dcdf5009c" . "context counts of genome assemblies by domain; not used for the verdict" . "" . . "Dataset: NCBI Datasets genome assembly counts" . . . . . "sha256:2dd9f3f3ded0ae3cb8f4276a4935e643c9118c9efbd879a23c7ab58fb6309fb3" . "sha256:56eee0562153075bbe9d33d9b0309394edef7defedc45ed47b69da29b96372f0" . "sha256:a3cb1bc5db0687820fbabf9102f4d42bac738923c65d20b7cf226b1c05225464" . . "delphisci claim tester (grok-4.7; cursor/darwin-varieties-life-tests-93ad)" . . . "grok-4.7" . . . . . . . . . "2026-11-08T16:24:31.860Z"^^ . . . . . "Claim test of darwin1859-ch14-04: refutes, confidence 0.46" . . "RSA" . "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAyFHtPB6p0mCjDozxlOY9CmO3nBm7Te3jFbXyLmM+G1tGe47Da0jYIbhkl9Oo1ZjxmCa3LCwlEqK8gj6hEIcL4ahW30JXh9HycfKVk1bugkzMEV15tJdTzAU3sGMFnbNsn43xY2LFLy45tApkRm5byEiM7oRRrooIdwoQKnP4Wzq/bfAoJ9qvW+MGkelyOTd1jbSt+KfmT7xKmDfWzAHddUlWwMX2QJOLxDaffOteTop6ZRgPiNmPJKA/S62Gac9XtSYT3oDkZ6/nr5cxxNLpOyxTf5FPISiCe11xYO/unYjY+mwnOfA8yhJIyx1l0O9I0vpOpn+E5TvyHJV+oSTrvwIDAQAB" . "fzNIh+bpKXr7X1KqAMI4loGBrAw3tI1PVELeRXt569cdKTuCkmhDsWiiFHG+i13ttfiWgWepbNXts0dvzNjqLZPeCM87qQbMnJhIInpFPWKm9neBxP1uF5N36Ume4yiAUaJ74ShuO6ZU9Oq+MSrxMmezgZmyogHcca8cpkxpJB8N7B+e17Vt9A1FjF0bqSMGzweCDTPoaObgvvAU4aj6agGBJREal1TbbHaHQC3ND9jO87QQeH7F6jvuHVPM4lbfVhdIgYeZgseBZ36khzigirfUEIeF83hfF00gtfCgEdO2CIgavD4Y6rlNB23SVUwJTlvZF2X30l4r3s0olhtTqg==" . . .